منابع مشابه
Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression
Vascular smooth muscle cells (VSMC) in the rat mesenteric artery show specific immunofluorescent staining with antisera against purified human uterine myosin (ASMM) but not human platelet myosin (APM). However, in primary cultures produced by enzymatic dissociation of this vessel, VSMC stain specifically with both ASMM and APM within 5 h after plating and throughout growth to confluence (4-10 d...
متن کاملRedistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy.
Immunofluorescence microscopy is widely used to characterize the cellular distribution of both soluble and structural proteins. Control experiments generally address only the specificity of the antibodies used. The permeabilization/fixation conditions used to prepare cells for antibody application are assumed to preserve faithfully the in vivo distributions of the protein(s) being examined. We ...
متن کاملMyosin in Cultured Vascular Smooth Muscle Cells" Immunofluorescence and Immunochemial Studies of Alterations in Antigenic Expression
Vascular smooth muscle cells (VSMC) in the rat mesenteric artery show specific immunofluorescent staining with antisera against purified human uterine myosin (ASMM) but not human platelet myosin (APM). However, in primary cultures produced by enzymatic dissociation of this vessel, VSMC stain specifically with both ASMM and APM within 5 h after plating and throughout growth to confluence (4-10 d...
متن کاملCultured human amniotic fluid cells characterized with antibodies against intermediate filaments in indirect immunofluorescence microscopy.
Cells cultured from second trimester human amniotic fluid were characterized in indirect immunofluorescence (IIF) microscopy using specific antibodies against the subunit proteins of different types of cytoskeletal intermediate filaments. Most of the amniotic fluid cell cultures contained only epithelial cells as indicated by the positive keratin-fluorescence in IIF. Five distinct types of kera...
متن کاملIndirect immunofluorescence tests with sera from African children and cultured Burkitt lymphoma cells.
In an earlier report (G. Henle and W. Henle, J. Bacteriol. 91:1248, 1966), results of immunofluorescence tests with cultured cells from Burkitt lymphomas were described, and evidence was presented that the small proportion of stainable cells might be those which harbor as yet unidentified herpeslike virus particles (M. A. Epstein et al., Wistar Inst. Symp. Monograph 4:69, 1965; S. E. Stewart et...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2015
ISSN: 2043-0116
DOI: 10.1038/protex.2015.021